Development and Validation of UV Spectrophotometric Method for Total Protein Estimation in Seeds of Pisum sativum L
نویسندگان
چکیده
A simple, accurate, precise and sensitive UV spectrophotometric method was developed for determination of total protein in seeds of Pisum sativum L. (Fabaceae). Phosphate buffer used as solvent and wavelength corresponding to maximum absorbance of the protein was found at 278.60nm. Beers law was observed in the concentration range of 2001000μg/ml with correlation coefficient 0.9996. The linear regression equation obtained by least square regression method were y=0.0008x + 0.0186, where y is the absorbance and x is the concentration of standard protein solution. The method was validated as per ICH guidelines. The values of relative standard deviation and % recovery were found to be satisfactory, indicating that proposed method is precise and accurate and hence can be used for the routine analysis of total protein estimation in seeds of Pisum Sativum L.
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